| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BERRY, Kade | SR | Winfield High School | 5:30.84 | Verified |
| 2 | BRANDT, Sadie | SR | St. Louis Blue Knights | 6:14.24 | Verified |
| 3 | BIBB, Dianna | SO | Bowling Green High School | 6:32.51 | Verified |
| 4 | BOSCHERT, Lily | SR | Winfield High School | 6:39.84 | Verified |
| 5 | AUER, Audrey | FR | Silex High School | 6:42.28 | Verified |
| 6 | LILLEY, Addison | FR | Bowling Green High School | 7:10.00 | Unverified: |
| 7 | HILLEBRAND, Daniella | JR | St. Louis Blue Knights | 7:29.91 | Verified |
| 8 | KUSMANOFF, Caroline | SR | St. Louis Blue Knights | 8:19.56 | Verified |
| 9 | LANE, Hanna | SR | Pacific High School | NT | Unverified: |
| 10 | STILES, Anya | SO | St. Louis Blue Knights | NT | Unverified: |
| 11 | MAZZEI, Lillian | SO | Silex High School | NT | Unverified: |
| 12 | HARTLEY, Ellie | FR | Elsberry High School | NT | Unverified: |
| 13 | HARKE, Gracin | FR | Silex High School | NT | Unverified: |
| 14 | JONES, Ruby | FR | Pacific High School | NT | Unverified: |
| 15 | DRYER, Grace | FR | Pacific High School | NT | Unverified: |