| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pacific High School | B | Avilez | Sheets | Zapolski | Avilez | NT | Unverified: |
| 3 | St. Louis Blue Knights | A | Kusmanoff | Moore | Gaugh | Mudge | NT | Unverified: |
| 4 | Bowling Green High School | A | Dunn | Eskew | Oberhaus | Huber | 1:43.00 | Unverified: |
| 5 | Pacific High School | A | Krug | Reeder | Thomas | Magsigay | NT | Unverified: |
| 6 | Winfield High School | A | Schulte | Stallard | Johnson | Geiss | DQ | Unverified: |
| 7 | Clopton High School | A | Adams | Jolly | West | Burt | NT | Unverified: |