| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bowling Green High School | A | Dawson | Gamm | Charlton | Dean | NT | Unverified: |
| 2 | Pacific High School | B | Abraham | Adair | Huerta | Stogsdill | NT | Unverified: |
| 3 | Pacific High School | A | Bonner | Reeder | Murphy | Blackburn | NT | Unverified: |
| 4 | Clopton High School | A | Bankhead | Palmer | Mitchell | Myers | 2:13.26 | Verified |
| 5 | Winfield High School | A | Reinebach | Stark | Pierre | Gillespie | NT | Unverified: |
| 6 | Silex High School | A | Kuntz | Weatherford | Hall | Thoroughman | NT | Unverified: |
| 7 | St. Louis Blue Knights | A | Etcher | Riggs | Knoerr | Plymale | NT | Unverified: |
| 8 | Elsberry High School | A | Howard | Talbot | Smith | Irving | NT | Unverified: |