| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Louis Blue Knights | A | Schark | Leine | Seth | Bryant | NT | Unverified: |
| 3 | Pacific High School | B | Sheets | Carrico | Stahl | Zapolski | NT | Unverified: |
| 4 | Bowling Green High School | A | Chance | Hughes | Griffith | Mix | 3:52.00 | Unverified: |
| 5 | Winfield High School | A | Hyde | Geiss | Shoemake | Schulte | NT | Unverified: |
| 6 | Bowling Green High School | B | Huber | Matlock | Soto | Oberhaus | NT | Unverified: |
| 7 | Pacific High School | A | Krug | Reeder | Wigger | Magsigay | NT | Unverified: |