| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | RIGGS, Charis | FR | St. Louis Blue Knights | NT | Unverified: |
| 3 | EDMONDS, Arionna | FR | Pacific High School | NT | Unverified: |
| 4 | VAN HORN, Austyn | FR | Silex High School | NT | Unverified: |
| 5 | PLYMALE, Cami | FR | St. Louis Blue Knights | NT | Unverified: |
| 6 | HOWARD, Chloe | FR | Elsberry High School | NT | Unverified: |
| 7 | PLYMALE, Cali | FR | St. Louis Blue Knights | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GRESHAM, Hannah | JR | St. Louis Blue Knights | 34.80 | Verified |
| 2 | TIPPIT, Julia | SO | Elsberry High School | 32.05 | Verified |
| 3 | GAMM, Avery | JR | Bowling Green High School | 31.00 | Verified |
| 4 | CHARLTON, Kaylyn | JR | Bowling Green High School | 30.42 | Verified |
| 5 | JOHNSON, Ava | FR | Bowling Green High School | 30.46 | Unverified: |
| 6 | DAWSON, Olivia | SO | Bowling Green High School | 31.00 | Unverified: |
| 7 | KERKEMEYER, Jozee | FR | Winfield High School | 33.21 | Verified |
| 8 | SHEPPARD, Reagan | FR | Winfield High School | 35.47 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BONNER, Layla | SO | Pacific High School | 29.00 | Verified |
| 2 | IRVING, Audrianna | JR | Elsberry High School | 28.85 | Verified |
| 3 | TALBOT, Joanna | SO | Elsberry High School | 28.67 | Verified |
| 4 | STARK, Evelyn | SO | Winfield High School | 27.59 | Verified |
| 5 | LAY, Alexis | JR | Pacific High School | 28.10 | Unverified: |
| 6 | WEATHERFORD, Sarah | JR | Silex High School | 28.82 | Verified |
| 7 | HALL, Maddy | SR | Silex High School | 28.91 | Verified |
| 8 | TALBERT, Stacia | JR | Clopton High School | 29.30 | Verified |