| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BERRY, Kade | SR | Winfield High School | 2:37.72 | Verified |
| 2 | BRYANT, Tabitha | SO | St. Louis Blue Knights | 2:38.72 | Verified |
| 3 | REINEBACH, Isabel | SO | Winfield High School | 2:44.57 | Verified |
| 4 | PICKENS, Emma | SR | Silex High School | 2:54.04 | Verified |
| 5 | BIBB, Dianna | SO | Bowling Green High School | 2:57.71 | Verified |
| 6 | BOSCHERT, Lily | SR | Winfield High School | 2:59.14 | Verified |
| 7 | SIMMONS, Sophie | FR | Clopton High School | 3:03.87 | Verified |
| 8 | LILLEY, Addison | FR | Bowling Green High School | 3:05.00 | Unverified: |
| 9 | BRANDT, Sadie | SR | St. Louis Blue Knights | 3:05.25 | Verified |
| 10 | THOROUGHMAN, Klaire | SO | Silex High School | 3:18.24 | Verified |
| 11 | EGGEMEYER, Victoria | JR | Clopton High School | 3:18.71 | Verified |
| 12 | TRUETKEN, Katherine | FR | Clopton High School | 3:18.75 | Verified |
| 13 | GRESHAM, Abi | JR | St. Louis Blue Knights | 3:34.86 | Verified |
| 14 | DRYER, Grace | FR | Pacific High School | NT | Unverified: |
| 15 | JONES, Ruby | FR | Pacific High School | NT | Unverified: |
| 16 | MURPHY, Ella | SR | Pacific High School | NT | Unverified: |
| 17 | AUER, Audrey | FR | Silex High School | NT | Unverified: |
| 18 | HARTLEY, Ellie | FR | Elsberry High School | NT | Unverified: |
| 19 | MAJORS, Emily | SO | St. Louis Blue Knights | NT | Unverified: |
| 20 | HARKE, Gracin | FR | Silex High School | NT | Unverified: |