| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BARTON, Nathaniel | SO | St. Louis Blue Knights | NT | Unverified: |
| 3 | ANDERSON, Reuben | FR | St. Louis Blue Knights | NT | Unverified: |
| 4 | TAPLEY, Austin | FR | Elsberry High School | NT | Unverified: |
| 5 | SZYMANSKI, Jeremiah | SO | St. Louis Blue Knights | NT | Unverified: |
| 6 | SCHELLMAN, Nathan | FR | St. Louis Blue Knights | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOLLY, Austin | SO | Clopton High School | 1:00.98 | Verified |
| 2 | MIX, Nathaniel | SO | Bowling Green High School | 49.00 | Unverified: |
| 3 | HYDE, Matthew | JR | Winfield High School | 43.78 | Verified |
| 4 | STARKS, Marcus | SR | Bowling Green High School | 41.55 | Verified |
| 5 | WOLF, Sach | JR | Pacific High School | 42.07 | Verified |
| 6 | JENNINGS, Matthew | SR | Bowling Green High School | 46.18 | Verified |
| 7 | BODE, Nickolas | SO | Pacific High School | 51.18 | Verified |
| 8 | SEITHEL, CAYDEN | SO | Silex High School | NT | Unverified: |