| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ABRAHAM, Marlene | JR | Pacific High School | NT | Unverified: |
| 2 | SCHELLMAN, Katherine | SR | St. Louis Blue Knights | NT | Unverified: |
| 3 | PLYMALE, Cami | FR | St. Louis Blue Knights | NT | Unverified: |
| 4 | ETCHER, Sara | JR | St. Louis Blue Knights | 1:26.97 | Verified |
| 5 | BROWN, Faith | FR | St. Louis Blue Knights | NT | Unverified: |
| 6 | HARTLEY, Ellie | FR | Elsberry High School | NT | Unverified: |
| 7 | PIERRE, Elsa | SR | Winfield High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAYNARD, Alexea | FR | Bowling Green High School | 1:25.00 | Unverified: |
| 2 | KORTE, Lucy | FR | Bowling Green High School | 1:20.00 | Unverified: |
| 3 | ISOM, Maddison | JR | Silex High School | 1:15.00 | Verified |
| 4 | IRVING, Audrianna | JR | Elsberry High School | 1:13.52 | Verified |
| 5 | BERRY, Layla | FR | Bowling Green High School | 1:14.00 | Unverified: |
| 6 | MITCHELL, Avenley | FR | Clopton High School | 1:16.32 | Verified |
| 7 | TRUETKEN, Katherine | FR | Clopton High School | 1:24.00 | Verified |
| 8 | SIMMONS, Sophie | FR | Clopton High School | 1:26.15 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TURNER, Reese | FR | Bowling Green High School | 1:09.97 | Verified |
| 2 | BONNER, Layla | SO | Pacific High School | 1:09.00 | Unverified: |
| 3 | HARDWICK, Stevajah | FR | Elsberry High School | 1:07.43 | Verified |
| 4 | DANIEL, Aleyna | SR | Pacific High School | 1:03.10 | Verified |
| 5 | REINEBACH, Grace | SR | Winfield High School | 1:03.82 | Verified |
| 6 | TALBERT, Stacia | JR | Clopton High School | 1:07.51 | Verified |
| 7 | KLEIN, Sydney | 8 | Silex High School | 1:09.89 | Verified |
| 8 | PICKENS, Emma | SR | Silex High School | 1:11.64 | Verified |