| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Winfield High School | A | Nelke | Stark | Reinebach | Reinebach | NT | Unverified: |
| 2 | Bowling Green High School | A | Charlton | Ortega | Johnson | Gamm | NT | Unverified: |
| 3 | Clopton High School | A | Talbert | Williamson | Palmer | Bankhead | 1:10.34 | Verified |
| 4 | Silex High School | A | Kuntz | Hall | Weatherford | Thoroughman | 55.52 | Verified |
| 5 | Pacific High School | B | Abraham | Huerta | Meyer | Stogsdill | 57.20 | Unverified: |
| 6 | St. Louis Blue Knights | A | Knoerr | Smith | Ward | Miller | NT | Unverified: |
| 7 | Elsberry High School | A | Tippit | Hardwick | Brinkman | Smith | NT | Unverified: |
| 8 | Winfield High School | B | Holder | Pierre | Gillespie | White | NT | Unverified: |