| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HENNINGFIELD, Elija | SO | Bowling Green High School | 5:02.00 | Verified |
| 2 | SOTO, Jose | SR | Bowling Green High School | 5:02.05 | Verified |
| 3 | TALLO, Mason | SO | St. Louis Blue Knights | 5:35.34 | Verified |
| 4 | GRADDY, Hunter | SO | Winfield High School | 5:45.60 | Verified |
| 5 | CHESSER, Noah | JR | Silex High School | 6:31.48 | Verified |
| 6 | ROBERT, Josiah | JR | Pacific High School | NT | Unverified: |
| 7 | NOXON, Joey | FR | Silex High School | NT | Unverified: |
| 8 | SPRAGGON, Joseph | FR | Winfield High School | NT | Unverified: |
| 9 | WIGGER, Sean | SO | Pacific High School | NT | Unverified: |
| 10 | SMITH, Ben | FR | Pacific High School | NT | Unverified: |
| 11 | CARRICO, Noah | SR | Pacific High School | NT | Unverified: |
| 12 | ANDERSON, Reuben | FR | St. Louis Blue Knights | NT | Unverified: |
| 13 | WAMSLEY, James | SO | St. Louis Blue Knights | NT | Unverified: |
| 14 | SANTOS, Alan | SO | Elsberry High School | NT | Unverified: |
| 15 | HERAS, Jedidiah | FR | Elsberry High School | NT | Unverified: |
| 16 | MILLER, Seth | SO | Elsberry High School | NT | Unverified: |
| 17 | BARFIELD, Joel | SO | St. Louis Blue Knights | NT | Unverified: |
| 18 | BYERS, Judah | FR | Elsberry High School | NT | Unverified: |