| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SINGER, Rachel | SR | Villa Duchesne High School | 2:31.00 | Verified |
| 2 | LOCHT, Emma | SO | Visitation Academy | 2:43.52 | Verified |
| 4 | LAURINE, Elizabeth | JR | Rosati-Kain High School | 3:00.51 | Verified |
| 5 | PFEIFFER, Eleanor | SO | Ursuline Academy | 3:01.07 | Verified |
| 6 | STEWART, Anna | JR | Ursuline Academy | 3:20.60 | Verified |
| 7 | VANNOY, Ryan | SR | Ursuline Academy | 3:23.00 | Verified |
| 8 | REED, Kathleen | SO | Visitation Academy | NT | Unverified: |
| 9 | BISHARA, Lola | FR | Visitation Academy | NT | Unverified: |