| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REED, Kathleen | SO | Visitation Academy | NT | Unverified: |
| 2 | SMITH, Amerynn | SO | Rosati-Kain High School | NT | Unverified: |
| 3 | TENG, Caroline | SR | Ursuline Academy | NT | Unverified: |
| 4 | ELSTON, Brooke | SR | Rosati-Kain High School | NT | Unverified: |
| 5 | MEYERS, Samantha | FR | Visitation Academy | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FISCHER, Sydney | FR | Ursuline Academy | 1:13.76 | Verified |
| 2 | WEIS, Emme | SO | Villa Duchesne High School | 1:06.51 | Verified |
| 3 | JANOSKY, Bennett | SO | Villa Duchesne High School | 1:04.21 | Verified |
| 4 | STRUCKEL, Lindsey | JR | Notre Dame (St. Louis) High Scho | 1:06.48 | Verified |
| 5 | BRYOWSKY, Niamh | FR | Ursuline Academy | 1:06.75 | Verified |
| 6 | LAURINE, Elizabeth | JR | Rosati-Kain High School | 1:14.97 | Verified |