| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ursuline Academy | A | Teng | Boresi | Fischer | Bryowsky | NT | Unverified: |
| 3 | Notre Dame (St. Louis) High Scho | A | Durandisse | McGill | Callico | Harris | 58.43 | Verified |
| 4 | Visitation Academy | A | Burgoon | Nunez | Lane-Liwo | Peoples | NT | Unverified: |
| 5 | Villa Duchesne High School | A | Irvin | Trevino | O'Daniel | Wilson | NT | Unverified: |