| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SINGER, Rachel | SR | Villa Duchesne High School | 5:38.37 | Verified |
| 2 | WENGER, Madeline | SO | Rosati-Kain High School | 6:16.41 | Verified |
| 3 | HAKE, Ava | JR | Notre Dame (St. Louis) High Scho | 6:40.11 | Verified |
| 4 | MACHISEN, Colleen | SR | Notre Dame (St. Louis) High Scho | 6:42.57 | Verified |
| 5 | LOCHT, Emma | SO | Visitation Academy | 6:48.76 | Verified |
| 6 | PFEIFFER, Eleanor | SO | Ursuline Academy | 6:51.97 | Verified |
| 9 | REDD, Bella | JR | Villa Duchesne High School | NT | Unverified: |
| 10 | KRAEMER, Heidi | JR | Visitation Academy | NT | Unverified: |