| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Charles West High School | A | Green | Montalvo | Parrish | Williams | 1:36.63 | Verified |
| 4 | Winfield High School | A | Schulte | King | Martin | Stallard | 1:36.44 | Verified |
| 5 | Ft. Zumwalt South High School | A | Beck | Butler | Pilger | Godefroid | 1:36.61 | Verified |
| 6 | Ft. Zumwalt East High School | A | Bailey | Booker | Glenn | Jones | 1:40.28 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Orchard Farm High School | A | Sarino | Medlock | Ruffing | Deaton | 1:36.05 | Verified |
| 2 | Warrenton High School | A | Dawson | Brosenne | Lombardo | Bristol | 1:35.10 | Verified |
| 3 | Timberland High School | A | Rushing | Owens | Sare | Matlick | 1:33.33 | Verified |
| 4 | Troy Buchanan High School | A | Freeman | Hopmann | Keene | LaRue | 1:32.00 | Verified |
| 5 | Hannibal High School | A | Essig | Turner | Bergheger | Williams | 1:32.14 | Verified |
| 6 | Bowling Green High School | A | Jennings | Starks | Dalton | Bryant | 1:33.50 | Unverified: |
| 7 | St. Clair High School | A | Bartz | Sanders | Furdek | Bekemeier | NT | Verified |
| 8 | St. Charles High School | A | Puati | Martinez | Nevels | Wilson | 1:36.42 | Verified |