| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Charles West High School | A | O'Kelley | Prost | Boxdorfer | Zeier | 1:58.25 | Verified |
| 3 | Orchard Farm High School | A | Jones | Pettit | Robinson | Jewell | NT | Verified |
| 4 | Hannibal High School | A | Brown | Stewart | LaJoy | Dean | 1:48.49 | Verified |
| 5 | Troy Buchanan High School | A | Ellison | Getz | Hunolt | Trower | 1:53.68 | Verified |
| 6 | Ft. Zumwalt South High School | A | Moller | Byrd | Krapf | Moore | 1:57.66 | Verified |