| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HENKE, Nathan | SR | Orchard Farm High School | 54.86 | Unverified: |
| 2 | FURDEK, Tyler | SO | St. Clair High School | 25.41 | Verified |
| 3 | BOOKER, Lerone | FR | Ft. Zumwalt East High School | 25.36 | Verified |
| 4 | SARINO, Colin | FR | Orchard Farm High School | 25.14 | Verified |
| 5 | PILGER, Whyett | SO | Ft. Zumwalt South High School | 25.20 | Verified |
| 6 | ORF, Easton | SO | Bowling Green High School | 25.36 | Verified |
| 7 | HAWKINS, Josh | SO | St. Clair High School | 25.86 | Verified |
| 8 | WILLIAMS, Aerion | JR | St. Charles West High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RICHARD, Seth | JR | St. Charles High School | NT | |
| 2 | FREEMAN, Myles | JR | Troy Buchanan High School | 24.14 | Verified |
| 3 | REEVES, Travis | SR | Timberland High School | 23.90 | Verified |
| 4 | BAILEY, Kristoff | SO | Ft. Zumwalt East High School | 23.66 | Verified |
| 5 | SAMS, Collin | JR | Winfield High School | 23.66 | Verified |
| 6 | RILEY, Bailey | JR | Winfield High School | 24.10 | Verified |
| 7 | DAWSON, Peyton | SO | Warrenton High School | 24.62 | Verified |
| 8 | PARRISH, Chance | SO | St. Charles West High School | 24.89 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PUATI, Sita | SR | St. Charles High School | 23.56 | Verified |
| 2 | BRYANT, Bleyne | JR | Bowling Green High School | 23.52 | Verified |
| 3 | TURNER, Dorian | JR | Hannibal High School | 23.01 | Verified |
| 4 | MOORE, Nicholas | SO | Troy Buchanan High School | 22.35 | Verified |
| 5 | WHITEHEAD, James | SR | Timberland High School | 22.65 | Verified |
| 6 | BERGHEGER, Sheldon | SR | Hannibal High School | 23.01 | Verified |
| 7 | BRISTOL, Zachariah | SR | Warrenton High School | 23.53 | Verified |
| 8 | BECK, Tony | SR | Ft. Zumwalt South High School | 23.62 | Verified |