| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WEAVER, Elinor | JR | Troy Buchanan High School | NT | Unverified: |
| 4 | MARTIN, Allyson | SR | St. Charles West High School | NT | Unverified: |
| 5 | MOORE, Alauna | SO | Ft. Zumwalt South High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HESTER, Hallee | SO | St. Charles High School | 33.04 | Verified |
| 2 | JEWELL, Dyamond | SR | Orchard Farm High School | 29.99 | Unverified: |
| 3 | SEBASTIAN, Mykenzy | FR | Warrenton High School | 29.42 | Verified |
| 4 | MOORE, Vada | SR | St. Clair High School | 28.21 | Verified |
| 5 | SCHATZLER, Cylee | SO | St. Clair High School | 29.35 | Verified |
| 6 | KULMA, Charlotte | FR | St. Charles High School | 29.83 | Verified |
| 7 | NICOLAY, Olivia | FR | Orchard Farm High School | 30.11 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REINEBACH, Grace | SR | Winfield High School | 27.97 | Verified |
| 2 | DEAN, Sameya | SO | Hannibal High School | 26.90 | Verified |
| 3 | SLEZAK, Paityn | FR | Timberland High School | 26.75 | Verified |
| 4 | PETERS, Reagan | FR | Troy Buchanan High School | 26.30 | Verified |
| 5 | MYERS, Makayla | SR | St. Charles West High School | 26.74 | Verified |
| 6 | GRAHAM, Knia | FR | Timberland High School | 26.80 | Unverified: |
| 7 | STEWART, Kyndall | SO | Hannibal High School | 27.82 | Verified |
| 8 | STARK, Evelyn | SO | Winfield High School | 28.20 | Verified |