| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIE, Arael | FR | Orchard Farm High School | NT | Unverified: |
| 2 | PAYNE, Avery | SO | Orchard Farm High School | 57.83 | Verified |
| 3 | SOMMER, Paige | FR | Warrenton High School | 54.48 | Verified |
| 4 | DANIEL, Kiera | SO | Warrenton High School | 53.92 | Verified |
| 5 | GETZ, Brynna | SO | Troy Buchanan High School | 53.96 | Verified |
| 6 | REESE, Makayla | JR | Ft. Zumwalt South High School | 55.59 | Verified |
| 7 | KERKEMEYER, Jozee | FR | Winfield High School | 1:03.71 | Verified |
| 8 | SKILLINGTON, Kierra | 9 | St. Charles High School | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROWN, Riyenn | SO | Hannibal High School | 53.72 | Verified |
| 2 | SCHATZLER, Cylee | SO | St. Clair High School | 51.23 | Verified |
| 3 | HARK, Nora | SR | Hannibal High School | 49.99 | Verified |
| 4 | SMITH, Hailey | SR | Timberland High School | 47.35 | Verified |
| 5 | NELKE, Jersy | SO | Winfield High School | 48.94 | Verified |
| 6 | DOUGLAS, Triniti | SR | Ft. Zumwalt South High School | 50.69 | Verified |
| 7 | ZEIER, Alivia | JR | St. Charles West High School | 52.74 | Verified |
| 8 | BROWN, Teryn | JR | Troy Buchanan High School | 53.82 | Verified |