| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BOSCHERT, Lily | SR | Winfield High School | NT | Unverified: |
| 3 | PRICHARD, Melissa | FR | St. Charles High School | 1:16.91 | Verified |
| 4 | WAELDER, Willa | SR | Hannibal High School | 1:11.64 | Verified |
| 5 | MORRIS, Isabelle | SO | Warrenton High School | 1:12.97 | Verified |
| 6 | BAER, Matilynn | SR | Orchard Farm High School | 1:21.95 | Verified |
| 7 | HOLDER, Megan | FR | Winfield High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBINSON, Jakai | FR | Orchard Farm High School | 1:06.78 | Verified |
| 2 | MYERS, Makayla | SR | St. Charles West High School | 1:05.11 | Verified |
| 3 | UTLEY, Sheridan | JR | Timberland High School | 1:04.52 | Verified |
| 4 | ADAMS, Alexa | SO | Timberland High School | 59.00 | Unverified: |
| 5 | MARSCHEL, Morgan | SO | Warrenton High School | 59.99 | Verified |
| 6 | JONES, Vivian | JR | St. Charles West High School | 1:05.01 | Unverified: Fort Zumwalt West Invite |
| 7 | LEWIS, Kalea | FR | Troy Buchanan High School | 1:05.76 | Verified |
| 8 | BALL, Jordan | SO | Hannibal High School | 1:08.21 | Verified |