| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ft. Zumwalt East High School | A | Bailey | Booker | Glenn | Jones | 48.40 | Verified |
| 4 | St. Charles West High School | A | Green | Buehrle | Montalvo | Parrish | 46.01 | Verified |
| 5 | Ft. Zumwalt South High School | A | Beck | Butler | Godefroid | Pilger | 46.37 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Clair High School | A | Bartz | Sanders | Bekemeier | Arndt | 45.32 | Verified |
| 2 | Warrenton High School | A | Dawson | Brosenne | Lombardo | Bristol | 45.11 | Verified |
| 3 | Timberland High School | A | Ervin | Reeves | Whitehead | Billings | 43.84 | Verified |
| 4 | Troy Buchanan High School | A | Freeman | Hopmann | Hall | Moore | 43.14 | Verified |
| 5 | Hannibal High School | A | Whiteside | Turner | Bergheger | Williams | 43.72 | Verified |
| 6 | Orchard Farm High School | A | Sarino | Medlock | Matlock | Henke | 44.87 | Verified |
| 7 | Winfield High School | A | Riley | Hyde | King | Martin | 45.20 | Verified |
| 8 | St. Charles High School | A | Lopez | Mbroh | Richard | Leech | 45.98 | Verified |