| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ft. Zumwalt East High School | A | Albers | Erickson | Davidson | Jackson | 5:16.75 | Verified |
| 4 | Ft. Zumwalt West High School | A | Laramie | McDaniel | Garcia | Anderson | NT | Verified |
| 5 | St. Charles High School | A | Prichard | Skillington | Fields | Hollrah | 4:34.27 | Verified |
| 6 | Ft. Zumwalt South High School | A | Barton | Pilliard | Layton | Hill | 5:25:00.00 | Unverified: Fort Zumwalt North Invitational |