| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | STALLARD, Alexavier | SO | Winfield High School | NT | Unverified: |
| 4 | SHOEMAKE, Cole | FR | Winfield High School | 28.61 | Verified |
| 5 | MAY, Gabriel | FR | Holt High School | 31.31 | Verified |
| 6 | REECE, Miles | FR | St. Charles High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TYLER, Travion | SO | Ft. Zumwalt West High School | 26.78 | Verified |
| 2 | HACKMANN, Jesse | JR | Warrenton High School | 26.59 | Verified |
| 3 | HENDERSON, Jacob | SO | Ft. Zumwalt West High School | 26.36 | Verified |
| 4 | BROSENNE, Gage | JR | Warrenton High School | 25.90 | Verified |
| 5 | MILLINER, Andre | FR | Ft. Zumwalt South High School | 26.19 | Verified |
| 6 | MARQUART, Tate | JR | St. Francis Borgia High School | 26.37 | Verified |
| 7 | DANIELS, Chris | FR | Holt High School | 26.74 | Verified |
| 8 | SANDERS, Joshua | JR | St. Charles High School | 26.91 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BOOKER, Lerone | FR | Ft. Zumwalt East High School | 25.36 | Verified |
| 2 | WILLIAMS, Macon | JR | Troy Buchanan High School | 24.96 | Verified |
| 3 | PILGER, Whyett | SO | Ft. Zumwalt South High School | 24.34 | Verified |
| 4 | WASHINGTON, Marvell | JR | St. Charles West High School | 23.40 | Verified |
| 5 | BAILEY, Kristoff | SO | Ft. Zumwalt East High School | 23.66 | Verified |
| 6 | CRAWMER, Ryan | SO | Troy Buchanan High School | 24.42 | Verified |
| 7 | POLITTE, Kolten | FR | St. Charles West High School | 25.29 | Verified |
| 8 | SCHMIEDER, Elijah | SO | St. Francis Borgia High School | 25.40 | Verified |