| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Charles High School | A | Adams | Wright | Fisdel | Sulwer | 8:16.56 | Verified |
| 2 | Ft. Zumwalt East High School | A | Nair | Parkes | Hoette | Jiang | 8:20.97 | Verified |
| 3 | Ft. Zumwalt West High School | A | Daugherty | Jimenez-Acosta | Ray | Schaeperkoetter | 8:28.93 | Verified |
| 4 | Ft. Zumwalt South High School | A | Wotawa | Rizo | Guetterman | Bates | 9:36.72 | Verified |