| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WEBER, Mattalyn | FR | St. Francis Borgia High School | 33.59 | Verified |
| 4 | STRAUB, Daylei | FR | Ft. Zumwalt South High School | 32.52 | Verified |
| 5 | STOWELL, Hailey | FR | Ft. Zumwalt West High School | 33.48 | Verified |
| 6 | SHEPPARD, Reagan | FR | Winfield High School | 35.47 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KORTE, Callie | SR | Holt High School | 32.34 | Verified |
| 2 | SMITH, Ella | FR | Ft. Zumwalt East High School | 31.59 | Verified |
| 3 | GIBBS, Destiny | FR | St. Charles West High School | 31.39 | Verified |
| 4 | JOHNSON, Arya | FR | Ft. Zumwalt West High School | 30.95 | Verified |
| 5 | MEDLING, Katie | FR | St. Charles West High School | 31.16 | Verified |
| 6 | RONDOT, Riley | SO | St. Francis Borgia High School | 31.43 | Verified |
| 7 | MOLLER, Kennedy | SO | Ft. Zumwalt South High School | 31.69 | Verified |
| 8 | HOLDER, Megan | FR | Winfield High School | 32.36 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PRICE, Elsie | FR | Troy Buchanan High School | 30.52 | Verified |
| 2 | REED, Lily | FR | Troy Buchanan High School | 30.37 | Verified |
| 3 | HESTER, Hallee | SO | St. Charles High School | 29.91 | Verified |
| 4 | SEBASTIAN, Mykenzy | FR | Warrenton High School | 29.42 | Verified |
| 5 | KULMA, Charlotte | FR | St. Charles High School | 29.83 | Verified |
| 6 | ADAMS, Irelyn | FR | Holt High School | 30.20 | Verified |
| 7 | SMITH, Grace | FR | Ft. Zumwalt East High School | 30.43 | Verified |
| 8 | SOMMER, Paige | FR | Warrenton High School | 30.93 | Verified |