| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SUTTON, Kenzie | SO | Ft. Zumwalt West High School | NT | Unverified: |
| 3 | HILL, Cortlynn | FR | Ft. Zumwalt South High School | 1:03.76 | Verified |
| 4 | MAYER, Kersten | SO | St. Francis Borgia High School | 1:01.87 | Verified |
| 5 | KERKEMEYER, Jozee | FR | Winfield High School | 1:03.71 | Verified |
| 6 | GEURIN, Morgan | FR | Warrenton High School | 1:04.15 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CHAUDHARI, Isha | SO | Ft. Zumwalt West High School | 1:00.16 | Verified |
| 2 | TUDOR, Sara | JR | Holt High School | 57.52 | Verified |
| 3 | SKILLINGTON, Kierra | FR | St. Charles High School | 54.95 | Verified |
| 4 | OXFORD, Savannah | FR | Ft. Zumwalt East High School | 52.81 | Verified |
| 5 | SOMMER, Paige | FR | Warrenton High School | 54.48 | Verified |
| 6 | ZEUSCHEL, Mariah | SO | Holt High School | 57.07 | Verified |
| 7 | UPTAIN, Marissa | FR | Ft. Zumwalt East High School | 59.39 | Verified |
| 8 | ALGAS, Denise | FR | Ft. Zumwalt South High School | 1:00.81 | Verified |