| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | CRAWMER, Ryan | SO | Troy Buchanan High School | NT | Unverified: |
| 4 | LEBISH, Gavin | SR | St. Francis Borgia High School | 1:07.48 | Verified |
| 5 | RICHARD, Brandon | SO | St. Charles West High School | NT | Unverified: |
| 6 | CRAVENS, Kirk | SO | Ft. Zumwalt East High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JIMENEZ-ACOSTA, Jaciel | FR | Ft. Zumwalt West High School | 1:03.05 | Verified |
| 2 | WILLIAMS, Teegan | FR | Warrenton High School | 1:01.56 | Verified |
| 3 | GLENN, Derrick | FR | Ft. Zumwalt East High School | 1:00.79 | Verified |
| 4 | ANZALDUA CAMPOS, Roy | FR | Ft. Zumwalt South High School | 1:00.65 | Verified |
| 5 | MILTON, Camren | SO | Ft. Zumwalt West High School | 1:00.67 | Verified |
| 6 | SHOEMAKE, Cole | FR | Winfield High School | 1:00.87 | Verified |
| 7 | COLEMAN, Dylan | SO | Warrenton High School | 1:02.02 | Verified |
| 8 | FILA, Owen | FR | Holt High School | 1:03.15 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DANIELS, Chris | FR | Holt High School | 1:00.08 | Verified |
| 2 | BATES, Milo | FR | Ft. Zumwalt South High School | 57.35 | Verified |
| 3 | JONES, Jackson | SO | St. Charles High School | 54.74 | Verified |
| 4 | SMITH, DeMarko | FR | St. Charles High School | 54.05 | Verified |
| 5 | GREEN, Javari | SO | St. Charles West High School | 54.74 | Verified |
| 6 | JONES, Brandon | JR | St. Francis Borgia High School | 57.21 | Verified |
| 7 | WRIGHT, Owen | FR | Troy Buchanan High School | 58.06 | Verified |
| 8 | STALLARD, Alexavier | SO | Winfield High School | 1:00.28 | Verified |