| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 5 | Ft. Zumwalt West High School | A | Snyder | McDaniel | McGrath | Anderson | NT | Verified |
| 6 | St. Charles High School | A | Skillington | Nottmeyer | Van Berkum | Fields | 11:03.85 | Verified |
| 7 | St. Francis Borgia High School | A | Schweich | Christiansen | Simon | Biondi | 11:14.61 | Verified |
| 8 | Ft. Zumwalt South High School | A | Harper | Rakowski | Holman | Barton | 11:23.21 | Verified |
| 9 | Holt High School | A | Hartman | Moore | Turley | Walker | 13:26.34 | Verified |