| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Point Middle School | A | Garrett | Mendoza | Palm | Mosher | 1:53.58 | Verified |
| 2 | Frontier Middle School | A | Lant | Rogers | Olson | Treadway | 1:50.05 | Verified |
| 3 | Wentzville South Middle School | A | Jones | Willis | clark | kirchhoff | 1:46.99 | Verified |
| 4 | Troy Middle School | A | Thompson | Amman | Croxton | Martin | 1:42.03 | Verified |
| 5 | Hannibal Middle School | A | 1:43.36 | Verified | ||||
| 6 | Troy South MS | A | Darrell | Toebben | Hunolt | Ryan | 1:48.52 | Verified |
| 7 | Black Hawk Middle School | A | Neeley | Morris | Samora | McPike | 1:50.21 | Verified |
| 8 | Wright City MS | A | Hamel | Schulte | Speiser | Rodriguez | 1:54.85 | Verified |