| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Black Hawk Middle School | A | Lorraine | Shoults | Duncan | Bennett | 2:07.81 | Verified |
| 4 | Wentzville South Middle School | A | Crider | Kaden | Hall | Sturma | 2:05.91 | Verified |
| 5 | Wright City MS | A | Jones | Jones | Meier | Wilson | 2:06.22 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Troy South MS | A | Enos | Coleman | Moore | Wilson | 2:03.87 | Verified |
| 3 | North Point Middle School | A | Edmonds | Nkombo | Goodson | Foster | 2:01.87 | Verified |
| 4 | Hannibal Middle School | A | 1:58.92 | Verified | ||||
| 5 | Troy Middle School | A | Benden | Hagen | Heimbach | Means | 1:59.56 | Unverified: |
| 6 | Wentzville Middle School | A | Zurmuehlen | Thompson | Moore | Lewis | 2:03.58 | Verified |
| 7 | Frontier Middle School | A | Caamano - Briones | Liddell | Griffith | De Los Reyes | 2:04.31 | Verified |