| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Black Hawk Middle School | A | Craven | Schmitt | Moreno | Pierce | 4:34.22 | Verified |
| 2 | Troy South MS | A | Workman | Weikel | Ryan | Ilgenfritz | 4:16.44 | Verified |
| 3 | Frontier Middle School | A | Malave | Lant | Olson | Williams | 4:12.94 | Verified |
| 4 | Troy Middle School | A | Bont | Martin | Thompson | Seymour | 4:00.12 | Verified |
| 5 | Hannibal Middle School | A | 4:02.71 | Verified | ||||
| 6 | North Point Middle School | A | Williams | Hall | Graham | Prieto | 4:13.44 | Verified |
| 7 | Wentzville South Middle School | A | Cooper | Dick | Medlock | kirchhoff | 4:20.60 | Verified |
| 8 | Wright City MS | A | Baugher | Bricker | Johnston | Staats | 4:42.27 | Verified |