| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wright City MS | A | Goodman | Ulery | Lopez-Luna | Manner | 5:30.86 | Verified |
| 2 | Troy South MS | A | Toedebusch | Arnold | Coleman | Raney | 4:58.28 | Verified |
| 3 | North Point Middle School | A | Edmonds | Rice | Sparkman | Foster | 4:40.01 | Verified |
| 4 | Troy Middle School | A | Witt | Pursifull | Bonds | Means | 4:36.17 | Verified |
| 5 | Hannibal Middle School | A | 4:38.39 | Verified | ||||
| 6 | Frontier Middle School | A | Caamano - Briones | Navarro Sanchez | Sauceda | De Los Reyes | 4:44.93 | Verified |
| 7 | Black Hawk Middle School | A | Lorraine | Bennett | Hopper | Gaffney | 5:09.83 | Verified |