| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Black Hawk Middle School | A | Neeley | Perkey | Samora | Tucker | 52.88 | Verified |
| 2 | North Point Middle School | A | Wilmesherr | Roby | Schottel | Heers | 51.68 | Verified |
| 3 | Wentzville South Middle School | A | Willis | clark | Jones | Maull | 51.03 | Verified |
| 4 | Troy Middle School | A | Thompson | Amman | Croxton | Seymour | 48.78 | Verified |
| 5 | Hannibal Middle School | A | 50.30 | Verified | ||||
| 6 | Wright City MS | A | Hamel | Rodriguez | Speiser | Strunk | 51.25 | Verified |
| 7 | Troy South MS | A | Christopherson | Toebben | Hunolt | Reynolds-Brandt | 52.74 | Verified |
| 8 | Frontier Middle School | A | Roberts | Pegg | Rogers | Treadway | 54.07 | Verified |