| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Frontier Middle School | A | Caamano - Briones | Liddell | Daniels | Griffith | 59.29 | Verified |
| 2 | Troy South MS | A | Enos | Coleman | Keene | Hacker | 57.89 | Verified |
| 3 | Wright City MS | A | Bertrand | Ferrell | Meier | Wilson | 57.33 | Verified |
| 4 | North Point Middle School | A | Goodson | Hacker | Foster | Bampton | 56.25 | Verified |
| 5 | Hannibal Middle School | A | 56.44 | Verified | ||||
| 6 | Troy Middle School | A | Benden | Hagen | Glimka | Dale | 57.42 | Verified |
| 7 | Black Hawk Middle School | A | Gaffney | Bennett | Mitchell | Lorraine | 58.53 | Verified |
| 8 | Wentzville South Middle School | A | Crider | Smith | Hall | Sturma | 1:00.18 | Verified |