| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ALLEN, Jordan | SR | Francis Howell North High School | 23.23 | Verified |
| 2 | WHITEHEAD, James | SR | Timberland High School | 22.80 | Verified |
| 3 | SEAMON, Drevyn | SO | Rock Bridge High School | 22.44 | Verified |
| 4 | HOOD, Casey | SR | Rock Bridge High School | 22.39 | Verified |
| 5 | MOORE, Nicholas | SO | Troy Buchanan High School | 22.74 | Verified |
| 6 | HOWARD, Malachi | SR | Blue Springs South High School | 22.63 | Verified |
| 7 | BALLARD, Devante | SR | Francis Howell High School | 23.22 | Verified |
| 8 | HAYWARD, Henry | JR | Truman High School | 23.39 | Verified |