| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ANTHONY, Tijaria | SO | Francis Howell Central High Scho | 27.24 | Verified |
| 2 | JONES, Ja'Kiyra | 9 | Battle High School | 27.06 | Verified |
| 3 | DIXON, Olivia | SR | Blue Springs High School | 25.50 | Verified |
| 4 | ROSS, Jasmine | SO | Blue Springs South High School | 25.34 | Verified |
| 5 | GRAHAM, Knia | 9 | Timberland High School | 26.41 | Verified |
| 6 | BROWN, Mattie | SR | Blue Springs High School | 26.64 | Verified |
| 7 | PETERS, Reagan | 9 | Troy Buchanan High School | 27.18 | Verified |
| 8 | SLEZAK, Paityn | 9 | Timberland High School | 27.39 | Verified |