| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ALLEN, Jordan | SR | Francis Howell North High School | 42.27 | Verified |
| 2 | MCAFEE, Montarion | JR | Blue Springs High School | 41.71 | Verified |
| 3 | JONES, Darius | JR | Ft. Zumwalt West High School | 40.83 | Verified |
| 4 | GRUBB, Derek | SR | Smith-Cotton High School | 38.99 | Verified |
| 5 | DEWITT, Xander | JR | Ft. Zumwalt South High School | 40.66 | Verified |
| 6 | OJO, Samson | JR | Battle High School | 40.98 | Verified |
| 7 | LARUE, Lucas | JR | Troy Buchanan High School | 41.80 | Verified |
| 8 | BROWNING, Branden | SR | Truman High School | 42.47 | Verified |