| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WASHINGTON, Madison | SO | Ft. Zumwalt West High School | 49.97 | Verified |
| 2 | BUTLER, Jordyn | SR | Battle High School | 48.11 | Verified |
| 3 | PARSONS, Grace | SR | Rock Bridge High School | 45.03 | Verified |
| 4 | PETERSON, Athena | SO | Hickman High School | 44.54 | Verified |
| 5 | ADAMS, Alexa | SO | Timberland High School | 46.93 | Verified |
| 6 | CANTWELL, Kinzley | JR | Blue Springs South High School | 47.80 | Verified |
| 7 | SMITH, Hailey | SR | Timberland High School | 49.22 | Verified |
| 8 | GONZALES, Amari | JR | Francis Howell High School | 50.38 | Verified |