| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GRIER, Lucy | SR | Francis Howell High School | 1:02.71 | Verified |
| 2 | JONES, Peyton | SO | Ft. Zumwalt North High School | 1:01.75 | Verified |
| 3 | STEMMONS, Sy'Rae | SO | Hickman High School | 1:00.85 | Verified |
| 4 | JACKSON, Ariana | JR | Blue Springs High School | 57.82 | Verified |
| 5 | PETERS, Reagan | 9 | Troy Buchanan High School | 1:00.93 | Verified |
| 6 | FREEMAN, Gretchen | JR | Blue Springs South High School | 1:01.48 | Verified |
| 7 | GREEN, Leilani | JR | Liberty (Wentzville) High School | 1:02.02 | Verified |