| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mehlville High School | A | TREVISANO | Burch | Roberts | Dizdarevic | 1:58.72 | Verified |
| 2 | Ritenour High School | A | Brantley | McGhee | Green-williams | French | 1:51.28 | Verified |
| 3 | Lafayette (Wildwood) High School | A | 1:50.08 | Verified | ||||
| 4 | Eureka High School | A | 1:46.25 | Verified | ||||
| 5 | Parkway West High School | A | Drake | Fotheringham | Watts | Love | 1:49.68 | Verified |
| 6 | Parkway South High School | A | Bacon | Riegelsberger | Ullom | Wilson | 1:51.19 | Verified |
| 7 | Oakville High School | A | Czuppon | Mueller | JONAS | BROWER | 1:53.11 | Verified |
| 8 | Fox High School | A | Schepers | Rogers | Carter | West | NT | Verified |