| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mehlville High School | A | Nava | Wilder | Muminovic | Malik | 2:17.04 | Unverified: |
| 2 | Parkway West High School | A | Josef | Phillips | McLeod | Sanders | 2:00.16 | Unverified: |
| 3 | Eureka High School | A | Williams | Conley | DEELE | Renner | 1:55.75 | Unverified: Kimball/Wintermeyer Girls Invite |
| 4 | Parkway South High School | A | Bell | Hilgendorf | Scott | Stanford | 1:51.19 | Verified |
| 5 | Oakville High School | A | Benignus | WALTON | Clark | Kadymova | 1:53.11 | Verified |
| 6 | Lafayette (Wildwood) High School | A | 1:59.21 | Unverified: | ||||
| 7 | Fox High School | A | Masters | Vitale | Birschbach | Schwalbe | 2:00.24 | Verified |
| 8 | Ritenour High School | A | Royal | Phillips | Gainey | Shaw | 1:52:00.00 | Unverified: |