| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BUFFORD, Corielle | SO | Ritenour High School | NT | Unverified: |
| 4 | BOND, Yvonna | SR | Mehlville High School | 22.05 | Verified |
| 5 | TYNDALL, Aniya | SO | Ritenour High School | 22.23 | Verified |
| 6 | HOWARD, Madelyn | SO | Eureka High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MU, Sarah | FR | Lafayette (Wildwood) High School | 21.08 | Verified |
| 2 | VANDERHOOF, Amara | FR | Eureka High School | 20.09 | Verified |
| 3 | KOMMU, Lasya | FR | Lafayette (Wildwood) High School | 19.91 | Verified |
| 4 | BOLFING, Gabi | SR | Eureka High School | 18.09 | Verified |
| 5 | FRAZIER, Alexandria | SO | Parkway South High School | 18.90 | Verified |
| 6 | DOHERTY, GRACE | SO | Lafayette (Wildwood) High School | 20.08 | Verified |
| 7 | NOTTINGHAM, Ella | FR | Eureka High School | 20.61 | Verified |
| 8 | NORTHCROSS, Demecha | SO | Parkway West High School | 21.59 | Verified |