| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | A | 9:36.15 | Verified | ||||
| 2 | Eureka High School | A | 10:12.69 | Verified | ||||
| 3 | Parkway South High School | A | Miele | Owens | Murphy | Lena | 10:29.55 | Verified |
| 4 | Parkway West High School | A | Mortensen | Paquette | Picaud | Etchason | 11:20.60 | Verified |
| 5 | Mehlville High School | A | Barrett | Escamilla | Green | Dizdarevic | 11:43.41 | Verified |
| 6 | Ritenour High School | A | BEARD | Chaidez | Weissler | Wood | 11:53.32 | Verified |