| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Parkway South High School | A | Ullom | Owens | Lena | Singleton | 4:26.46 | Verified |
| 3 | Parkway West High School | A | Drake | Fotheringham | Love | Watts | 4:20.32 | Verified |
| 4 | Lafayette (Wildwood) High School | A | 4:13.00 | Verified | ||||
| 5 | Eureka High School | A | 4:13.41 | Verified | ||||
| 6 | Oakville High School | A | Czuppon | WESCOAT | BROWER | JONAS | 4:22.70 | Verified |
| 7 | Ritenour High School | A | Brantley | Bell | Green-williams | McGhee | 4:31.79 | Verified |