| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ritenour High School | A | Henderson | Porter | Tyndall | Gainey | 4:32:00.00 | Unverified: |
| 2 | Parkway West High School | A | Josef | Phillips | Sanders | Masalskis | 4:45.00 | Unverified: |
| 3 | Eureka High School | A | Barsh | BRICKMAN | DAFFRON | Contestabile | 4:31.49 | Unverified: Kimball/Wintermeyer Girls Invite |
| 4 | Oakville High School | A | Benignus | WALTON | Clark | Kadymova | 4:22.70 | Verified |
| 5 | Parkway South High School | A | Bell | Gavornik | McHale | Stanford | 4:26.46 | Verified |
| 6 | Lafayette (Wildwood) High School | A | 4:40.00 | Unverified: | ||||
| 7 | Mehlville High School | A | Roberts | Otajagic | Dang | Mask | 5:46.09 | Unverified: |