| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ASHOK, Riya | JR | Parkway West High School | 30.60 | Verified |
| 2 | SADDLER, Kaliyah | SR | Lafayette (Wildwood) High School | 29.24 | Verified |
| 3 | O'SHEA, Jenna | SR | Mehlville High School | 28.69 | Verified |
| 4 | STROOT, Clara | FR | Parkway South High School | 28.38 | Verified |
| 5 | TROMPETER, Aleah | JR | Fox High School | 28.38 | Verified |
| 6 | JONAS, MADYSEN | JR | Oakville High School | 28.84 | Verified |
| 7 | SCHEPERS, Michelle | SR | Fox High School | 30.55 | Verified |
| 8 | TREVISANO, TAYLOR | SR | Mehlville High School | 31.22 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOHNSON, Nykira | JR | Parkway West High School | 28.26 | Verified |
| 2 | BACON, Lauren | SO | Parkway South High School | 28.12 | Verified |
| 3 | JONES, Talia | SO | Ritenour High School | 27.30 | Verified |
| 4 | BROWN, Avery | SO | Lafayette (Wildwood) High School | 26.42 | Verified |
| 5 | MITCHELL, Hannah | JR | Eureka High School | 27.08 | Verified |
| 6 | BRANTLEY, Chayl | JR | Ritenour High School | 27.86 | Verified |
| 7 | MUELLER, Jenna | SR | Oakville High School | 28.14 | Verified |
| 8 | DOSS, MADISON | SO | Eureka High School | 28.27 | Verified |