| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PAULS, Lauren | SO | Lafayette (Wildwood) High School | 2:20.66 | Verified |
| 2 | PEARCE, OLIVIA | SO | Eureka High School | 2:25.89 | Verified |
| 3 | GROENEWALD, Mizelle | SO | Lafayette (Wildwood) High School | 2:29.39 | Verified |
| 4 | CZUPPON, Addison | JR | Oakville High School | 2:29.66 | Verified |
| 5 | ETCHASON, Alexandra | SR | Parkway West High School | 2:31.34 | Verified |
| 6 | ETCHASON, Sydney | SO | Parkway West High School | 2:34.61 | Verified |
| 7 | RAKEY, Madeline | FR | Eureka High School | 2:35.28 | Verified |
| 8 | MIELE, Allison | FR | Parkway South High School | 2:36.82 | Verified |
| 9 | BROWER, EMILIE | SO | Oakville High School | 2:39.53 | Verified |
| 10 | BARRETT, Kayla | SO | Mehlville High School | 2:39.62 | Verified |
| 11 | MILLER, Allison | SO | Mehlville High School | 2:41.42 | Verified |
| 12 | MURPHY, Sophia | SO | Parkway South High School | 2:42.31 | Verified |
| 13 | WOOD, Johanna | JR | Ritenour High School | 2:51.30 | Verified |
| 14 | CHAIDEZ, Denisse | SO | Ritenour High School | 2:54.07 | Verified |