| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KIRBY, Kennedi | JR | Eureka High School | 50.64 | Verified |
| 2 | O'SHEA, Jenna | SR | Mehlville High School | 50.37 | Verified |
| 3 | GENTILINI, Olivia | SR | Eureka High School | 49.62 | Verified |
| 4 | WATTS, Cecelia | SR | Parkway West High School | 47.83 | Verified |
| 5 | FRENCH, Mikel | SR | Ritenour High School | 49.27 | Verified |
| 6 | WITHERSPOON, Layne | SR | Lafayette (Wildwood) High School | 50.17 | Verified |
| 7 | WEISS, Makenna | JR | Lafayette (Wildwood) High School | 50.39 | Verified |
| 8 | BANKS, Tristen | JR | Parkway West High School | 51.49 | Verified |