| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NORTHCROSS, Demecha | SO | Parkway West High School | 1:31.86 | Verified |
| 2 | GAVORNIK, Ashlyn | SR | Parkway South High School | 1:03.89 | Verified |
| 3 | FRAZIER, Alexandria | SO | Parkway South High School | 1:01.01 | Verified |
| 4 | BUFFORD, Corielle | SO | Ritenour High School | 1:00.56 | Verified |
| 5 | PORTER, Whitney | FR | Ritenour High School | 1:00.75 | Verified |
| 6 | WHEELER, Julia | SO | Mehlville High School | 1:02.30 | Verified |
| 7 | BOLFING, Gabi | SR | Eureka High School | 1:05.56 | Verified |
| 8 | HOWARD, Madelyn | SO | Eureka High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BIRSCHBACH, Elayna | FR | Fox High School | 59.14 | Verified |
| 2 | NOTTINGHAM, Ella | FR | Eureka High School | 57.57 | Verified |
| 3 | KOMMU, Lasya | FR | Lafayette (Wildwood) High School | 57.47 | Verified |
| 4 | HENDERSON, Aniyah | FR | Ritenour High School | 53.56 | Verified |
| 5 | DOHERTY, GRACE | SO | Lafayette (Wildwood) High School | 55.16 | Verified |
| 6 | MU, Sarah | FR | Lafayette (Wildwood) High School | 57.49 | Verified |
| 7 | TYNDALL, Aniya | SO | Ritenour High School | 58.67 | Verified |
| 8 | BOND, Yvonna | SR | Mehlville High School | 59.36 | Verified |