| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | IVANOVA, Betina | SR | Parkway West High School | 1:10.94 | Verified |
| 3 | ASHOK, Riya | JR | Parkway West High School | 1:08.84 | Verified |
| 4 | WESCOAT, ELIZABETH | SO | Oakville High School | 1:08.03 | Verified |
| 5 | GREEN-WILLIAMS, Mariah | FR | Ritenour High School | 1:08.73 | Verified |
| 6 | BROWN, Janya | JR | Lafayette (Wildwood) High School | 1:09.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SQUIRES, AYDAN | JR | Oakville High School | 1:07.33 | Verified |
| 2 | OWENS, Annelise | SO | Parkway South High School | 1:05.70 | Verified |
| 3 | ULLOM, CiCi | JR | Parkway South High School | 1:03.37 | Verified |
| 4 | SCHNURBUSCH, Raegan | JR | Eureka High School | 1:00.97 | Verified |
| 5 | TROMPETER, Aleah | JR | Fox High School | 1:02.85 | Verified |
| 6 | SINGH, Shrutika | SR | Lafayette (Wildwood) High School | 1:03.60 | Verified |
| 7 | PALMER, Riley | SR | Mehlville High School | 1:06.25 | Verified |
| 8 | MORSE, Kathryn | SR | Eureka High School | 1:07.96 | Verified |